Thermo Scientific Mr. Frosty Freezing Container, Polycarbonate, Screw Cap, 117 × 86 mm, Holds 18 × 1.0–2.0 mL Tubes

The Thermo Scientific Mr. Frosty Freezing Container, Polycarbonate, Screw Cap, 117 × 86 mm, Holds 18 × 1.0–2.0 mL Tubes is designed for controlled cooling of cell samples during cryopreservation. Using isopropyl alcohol and a mechanical freezer, it provides a cooling rate close to 1°C per minute, supporting the preparation of frozen cell stocks before transfer to long-term storage.

Key Features:

• Brand: Thermo Scientific
• Tube Capacity: Holds 18 cryogenic tubes with individual capacities of 1.0 to 2.0 mL
• Controlled Cooling: Designed to reduce sample temperature by approximately 1°C per minute
• Construction: Polycarbonate body with a screw cap that helps retain tubes if the container tips accidentally
• Dimensions: 117 mm diameter × 86 mm height for a compact freezer footprint
• Label Protection: Keeps tubes separate from the alcohol, helping preserve labels and handwritten identification
• Application: Cell freezing for cryopreservation workflows in cell culture and research laboratories

Choose this reusable freezing container for a straightforward way to incorporate gradual cooling into routine cell preservation. It works with a mechanical freezer and an alcohol reservoir, making it practical for laboratories preparing cell stocks for future experiments.

Frequently Asked Questions

1. What is the Thermo Scientific Mr. Frosty Freezing Container used for?

It provides gradual cooling when freezing cultured cells for cryopreservation. Cells should be prepared in an appropriate freezing medium according to their specific protocol, then frozen before transfer to suitable long-term storage. Freezing conditions and medium selection should match the cell type being preserved.

2. Which tube sizes does the Thermo Scientific Mr. Frosty accommodate?

This version accommodates 18 cryogenic tubes with individual capacities between 1.0 and 2.0 mL. The stated range refers to tube capacity, so the sample fill volume should follow the selected tube’s instructions and the relevant cell freezing protocol.

3. What is required to use the Thermo Scientific Mr. Frosty?

The system requires 100% isopropyl alcohol and a mechanical freezer operating between -70°C and -80°C. The manufacturer instructs users to fill the alcohol reservoir to the marked line, load the sample tubes and leave the container undisturbed in the freezer for at least four hours.

4. How should the Thermo Scientific Mr. Frosty be maintained between freezing cycles?

Replace the isopropyl alcohol every fifth use and keep the container at room temperature when it is not being used. Clean it with a non-abrasive, non-alkaline detergent. These maintenance steps support reuse in subsequent freezing cycles.